Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
نویسندگان
چکیده
منابع مشابه
Direct detection of Mycobacterium tuberculosis rifampin resistance in bio-safe stained sputum smears
Direct smear microscopy of sputum forms the mainstay of TB diagnosis in resource-limited settings. Stained sputum smear slides can serve as a ready-made resource to transport sputum for molecular drug susceptibility testing. However, bio-safety is a major concern during transport of sputum/stained slides and for laboratory workers engaged in processing Mycobacterium tuberculosis infected sputum...
متن کاملAllele-specific rpoB PCR assays for detection of rifampin-resistant Mycobacterium tuberculosis in sputum smears.
We describe an allele-specific PCR assay to detect mutations in three codons of the rpoB gene (516, 526, and 531) in Mycobacterium tuberculosis strains; mutations in these codons are reported to account for majority of M. tuberculosis clinical isolates resistant to rifampin (RIF), a marker of multidrug-resistant tuberculosis (MDR-TB). Three different allele-specific PCRs are carried out either ...
متن کاملEarly and Efficient Detection of Mycobacterium tuberculosis in Sputum by Microscopic Observation of Broth Cultures
Early, efficient and inexpensive methods for the detection of pulmonary tuberculosis are urgently needed for effective patient management as well as to interrupt transmission. These methods to detect M. tuberculosis in a timely and affordable way are not yet widely available in resource-limited settings. In a developing-country setting, we prospectively evaluated two methods for culturing and d...
متن کاملApplication of Hyperbranched Rolling Circle Amplification for Direct Detection of Mycobacterium Tuberculosis in Clinical Sputum Specimens
BACKGROUND Global tuberculosis (TB) control is encumbered by the lack of a rapid and simple detection method for diagnosis, especially in low-resource areas. An isothermal amplification method, hyperbranched rolling circle amplification (HRCA), was optimized to detect Mycobacterium tuberculosis (Mtb) in clinical sputum specimens. METHODS A clinical validation study was performed to assess the...
متن کاملEvaluation of the FASTPlaqueTB assay for direct detection of Mycobacterium tuberculosis in sputum specimens.
SETTING Sputum samples were collected from suspected tuberculosis patients attending out-patient clinics at the Ojha Institute of Chest Diseases, Karachi, Pakistan. OBJECTIVE To evaluate the performance of the FASTPlaqueTB assay for rapid diagnosis of pulmonary tuberculosis. DESIGN A comparative study of 584 sputum samples using acid-fast smear microscopy, Lowenstein-Jensen culture and FAST...
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ژورنال
عنوان ژورنال: Revista da Sociedade Brasileira de Medicina Tropical
سال: 2018
ISSN: 1678-9849,0037-8682
DOI: 10.1590/0037-8682-0238-2017